COlSTDITIOlSrS FATOTJRING FEEMENTATIOTST. 



39 



formation of urea into carbonate of ammonia goes on at a very- 

 appreciable rate whilst the urine is exposed in the incubator to a 

 temperature of 122° F. (50° C). 



Experiments made with another specimen of urine of very nearly 

 the same acidity yielded the following results. Exactly one fluid- 

 ounce was used, as before, in each experiment. 



Urine whose aci- 

 dity was exactly 

 neutralized by ix 

 minims of liquor '' 

 potassiB to thefluid 

 ounce. 



{ Treatment. 



1 . Boiled gently for 5 ' 'over 

 flame, without spurting. 



2. Boiled for 2" over flame, 

 8" in can of water. 



3. Boiled for 2 ' 'over flame, 

 8" in can. Left at 122° F. 

 for 3 days. 



4. Boiled for 2"overflamei 

 18" in can. 



5. Boiled for 2" over flame, 

 \, 38" in can. 



Eesult. 



1. Diminished by 5i. 

 Acidity =m. vj|^ of liq. pot. 



2. Diminished about 3^. 

 Acidity =m. vj of liq. pot. 



3. Diminished by 3J. 

 Acidity =m. iv of liq. pot. 



4. Diminished by 5^^. 

 Acidity =m. iv§ liq. pot. 



5. Diminished by 3|-. 

 Acidity =m. iij liq. pot. 



The urea in this specimen seemed to undergo change rather 

 more rapidly than in the last, as a careful comparison of the 

 figures will indicate. Experiments 2, 4, 5 also seemed to show 

 that the change takes place most rapidly at first, and subsequently 

 tends to diminish ; thus in No. 2, after ten minutes' boiling over 

 flame and in can, we get a diminution of acidity equivalent to 

 m. iij of liquor potassse ; in Ko. 4, with an extra 10 minutes' boil- 

 ing in the can the further diminution of acidity only equals m. i| 

 of liquor potassse ; whilst in No. 5, with a still further period of 

 20 minutes' boiling the additional diminution of acidity was also 

 equivalent to m. i| of liquor potassse. 



Other experiments were made to ascertain whether the change 

 of urea into carbonate of ammonia would still go on at lower tem- 

 peratures ; and this time tbe urine was one of much higher acidity 

 and specific gravity. Exactly one fluid-ounce was used, as before, 

 in each experiment. 



Urine whose aci- 

 dity was exactly 

 neutralized by 

 m. xxj of liquor po- 

 tass^ to the fluid- 

 ounce. 



( Treatment. 



1. Boiled for 2"over flame, 

 8" in can. 



2. Boiled for 2"over flame, 

 8" in can. Left at 83° F. 

 for 7 days. 



3. Boiled for 2"over flame, 

 8" in can. Left at 122'' F. 



V for 7 days. 



Bssults. 



1. Acidity=m.xviijofliq. 

 pot. 



2. Acidity =m. xvj of liq. 

 pot. 



3. Acidity =m. xiij of liq. 

 pot. 



Erom this it appears that the diminution of acidity goes on at 



