314 MR. C. WARBURTON AND MISS A. L. EMBLETON ON 
infested bushes, cut into small fragments, and placed in tubes of 
spirit. These were shaken vigorously and then allowed to stand, 
and the sediment examined under the microscope. 
Eggs were first found in the buds removed on June 26. After 
that date they occurred in increasing numbers. By the end of 
the first week of July the new attack appeared to be firmly 
established. Mites in all stages of development, as well as eggs, 
ould easily be found by dissection of the new buds, some of 
which seemed to show sigus of abnormal development. In every 
case the mites were centrally situated in the buds—a remarkable 
fact in view of their particularly compact structure in this plant, 
which would render it by no means easy for the immigrant mites 
to reach the interior. 
As the brood increased, the mites worked centrifugaliy, 
‘encroaching gradually upon the more external portions of the bud. 
By July 20 the removal of two outside leaves sufficed, in some 
cases, to reveal the mites, and by the end of the month they were 
almost external in the most advanced buds, covered only by the 
loosely applied outermost leaves, while occasionally one or two 
individuals were found actually on the outside of the buds. 
The multiplication of the mites in the new buds had been so 
rapid during July and August, that it appeared highly probable 
that a second migration period was approaching. None such, 
however, was observed. The buds continued to swell, but re- 
mained green, and did not burst, and no wandering mites were 
detected during the autumn. The creatures were now established 
in their winter-quarters and were reproducing less actively. No 
new fact was to be added to the record of their life-history. 
Some might succumb to the rigours of winter, but the survivors 
would be ready to recommence the life-cycle in the following 
spring. 
Animals associated with the Mite. 
Runnicg with great activity over the twigs of infested bushes, 
examples of a red mite of the genus Actineda were constantly 
found. No conclusive evidence of its habits was obtained, but 
one specimen had attached to its jaws what appeared to be empty 
Hriophyes-skins, and it is quite possible that it preys to some 
extent on the mite, for it belongs to a predaceous group of the 
Trombidiide. 
Within the diseased buds specimens of a Tyroglyphus mite were 
