13 



Largely from the basis of the preceding information the writer 

 attempted to produce mature seeds in H. fiilva. The experi- 

 ments conducted divided themselves into four groups : 



I. Fertilization of the flowers with their own pollen. 

 a. After the blossoms had completely opened. 



h. Before the blossoms had opened sufficiently for them to be 

 pollinated from other sources, but when their own pollen seemed 

 about to discharge. 



c. Before the blossoms had opened sufficiently to obtain pollen 

 from other sources, an incision was made in the ovary, and pollen 

 as obtained in the preceding placed therein. 



d. Before the blossom had completely opened the stigma was 

 snipped off, and pollen placed directly upon the top of the style. 



II. Fertilization of the flower with pollen from the same clump 

 of day lilies. In this group further procedure was essentially 

 similar to that outlined in Group I. except that the anthers of the 

 flower were first removed. 



III. Fertilization of the flowers with the pollen of a far re- 

 moved clump of day lilies. Inquiry revealed the fact that this 

 group of the plants and that used in Group I did not have a com- 

 mon original locality and were probably genetically distinct. In 

 Group III also the further procedure was similar to Group I, ex- 

 cept that the anthers of the pollinated flower were first removed. 



IV. Fertilization of the flowers w^th pollen from the yellow 

 lily, H. flava. Attempts at cross-fertilization were made as al- 

 ready indicated in the other groups. 



Control of the above experiments was obtained by tying a small 

 paper bag over each experimental flower. The results of the 

 above experiments ivere negative in ever\ respect. No mature 

 seeds were ever formed. An effect of the paper bags was to 

 lengthen the life of the flower appreciably. 



In a previous number of Torreya (Vol. i8, Dec. 1918), double 

 flowers were reported for H. fiilva. Continued observation indi- 

 cates that such seem common in the Mississippi river region from 

 Missouri up to St. Paul, Minn. In some localities no other type 

 of flower was observed. N. M. Grier 



Washixgtox and Jefferson College, 

 Washington, Pa. 



