102 BENEFICIAL ACTION OF CERTAIN "POISONS"; AND 



water one in twenty-five. This — the control — was kept at 

 40°C and examined from time to time with the spectroscope 

 to ascertain the exact moment at which the oxyhaemoglo- 

 bin became reduced to haemoglobin. The other tube con- 

 tained poisoned liver-juice and blood, the juice and the poison 

 having been kept in contact for ten minutes before 

 being added to the blood. Some of the deadliest protoplasmic 

 poisons had very little retarding effect on hepatic reductase, 

 in fact caffeine (the citrate), in 0.01 normal solution prolonged 

 the time of reduction to 24.5 minutes, whereas hyoscine 

 hydrobromide of the same strength only prolonged it to 6.5 

 minutes, the normal time for the control being -1.5 minutes. 

 Some of our results are given in full in the table below; 



TABLE I. 



4.5 minutes required for reduction of normal mixture. 

 Time in minutes required for the reduction of mixtures 

 containing Ice of liver juice, 2cc of blood solution and Ice of 

 an aqueous solution of the poison having a normal concen- 

 tration of 



Poison 



0.1 



0.02 0.01 



0.0010.0001 



0.00001 



Hyoscine hj^dro 

 bromide 



Cocaine hydro- 

 chloride 



Morphine sulphate . 



Atropine sulphate. . 



Strychnine sulphate 



Quinine hydro- 

 chloride 



Caffeine citrate .... 



Alcohol 



Ether 



Chloroform 



5.5 



5. 5 

 6.0 



8.0 

 destroys 

 blood 



7.5 



8.0 

 7.0 

 6.0 



8.0 



6.5 



8.5 

 7.5 

 6.5 



6.0 



4.0 



10.0 

 6.5 



3.5 



4.5 

 7.5 



4.5 



4.5 



destroys 

 blood 

 10.0 



11. Oi 9.0 



I 



25.0J24.5 

 .. ..,10.0 

 5.0.. 

 10.0 . 



6.5 



8.0 



12.5 



6.5 

 9.0 



10.0 

 9.0 



5.0 



9.0 8.0 



6.0 

 0.9 



7.0 



