85 



some writers would limit the term to the restoration of embryonic 

 tissue in root and shoot, others would include within the scope of 

 the process merely the development of buds present before injury. 

 It seemed best to take the middle ground and consider as a regen- 

 eration any organ formed anew after injury or loss. 



The different plant organs were used as cuttings and their be- 

 havior examined when buds were absent. On the roots which 

 formed shoots it was found that these were not confined to the 

 upper (basal) surface, but could appear from the apical as well, 

 or from the middle of the root. The roots of less than half of 

 the species used formed shoots, while all produced roots not 

 always as true regenerations, but as outgrowths from the unin- 

 jured cambium. Budless stems proved able to root with ease 

 but were unable to replace the buds which had been cut out. 

 Such parts continued growing for fifteen months without under- 

 going any tissue change, while a part on which a single bud was 

 left established secondary vascular strands between the bud and 

 the new roots. The pseudobulb of an orchid proved able to re- 

 generate roots and a shoot from the base, and in a conifer the 

 apparent " restoration" of a single root on the seedling and in an 

 older stem-part was described. Of eighty-two species of leaves 

 used in experimentation only two new ones were found which 

 produced a shoot, though the large majority formed roots. Mod- 

 ified leaves of various types, such as phyllodes and bulb-scales, 

 were also found to be able to root. Regeneration was likewise 

 reported in the inflorescence of Dudley a calif or nica and Riiellia 

 rosea, in the fruits o{ Phaseolus vulgaris and P. lunatus, and finally in 

 the "head" of the alga Penicilliis capitatus. 



An extended discussion followed. 



Owing to the lateness of the hour. Dr. Rusby did not present 

 his paper on " Field Observations of the Past Year," but exhibited 

 a few interesting plants collected at Oscoda, Michigan. 



Dr. Southwick exhibited several interesting specimens of the 

 seeds of Ricinus. 



The meeting adjourned at ten o'clock. 



C. Stuart Gager, 



Secretary, 



