ural sequence after having made new roots and shoots. Of the four 

 pieces the basal made roots from its basal part and leaves from 

 its apical part while the next piece, which was Y-shaped, made 

 shoots from its basal part and roots from both its apical parts. 

 Of the two terminal pieces, one made roots from its basal and 

 shoots from its apical part while the other made shoots from its 

 basal and roots from its apical part. In all cases roots arose in 

 the water and shoots in the air. 



The effect of water and of air was again shown in the case of 

 a piece an inch and a half thick and nearly three feet long, which 

 had put out roots from the basal part in water and shoots from 

 the apical parts in air, but subsequently when so placed that 

 water dripped upon its apex and ran down its entire length with- 

 out accumulating at the base, put out new shoots amidst the 

 basal roots and new roots amidst the apical shoots. 



To see how^ very short lengths of pieces would behave, a few 

 pieces were cut 32 mm. wide and from 8 to 65 mm. long. These 

 were floated in shallow water, some base upward others apex 

 upward. Those 65 mm. long made leaves in the air and roots 

 in the water no matter which end was up. Some 17 mm. long 

 made only leaves. One very short disk 32 mm. wide and 8 mm. 

 deep put out one slight beginning of a shoot and of a root and 

 here the root was above the shoot and toward the apex end. 

 After many days these experiments were obscured by the drying 

 out of the water. 



Since roots were so readily made in the presence of water and 

 shoots in the presence of air, some twigs were hung up in moist 

 air after the method of Vochting but with access of light and 

 exclusion of free circulation of air. Hanging vertically, nearly, 

 in moist air, these twigs might be expected to show any polarity 

 they possessed, without the masking effects of the strong stimuli, 

 water and air, applied in most of the above experiments at oppo- 

 site poles. 



Sticks 10 mm. thick and 60 cm. long were cut into three and 

 four pieces, i 5 to 20 cm. long and tied together in bundles so 

 that successive pieces of each stick had base and apex downward 

 alternately. The bundles were hung nearly vertically in a bell- 



