142 



sugar maple stump was infected from the butternut. The black 

 ash log lies about one fourth of a mile north of the butternut, 

 over one hill. 



In the north part of the village of Ballston Spa, on the trunk 

 of some cultivated willow, July 20, 1912, was seen from the 

 railroad coach, what appeared to be punks of Polyporus ad- 

 mirabilis. Possibly, if this had been examined, it would have 

 been similar to Polyporus Underwoodii found by Dr. H. J. Banker, 

 Aug. 27, 1908, at Schaghticoke, Rensselaer county. 

 Hudson Falls, N. Y. 



SHORTER NOTES 



A Note on Preservatives for Algae. In The Plant World 

 for August, 1913, is an article by Gilbert Morgan Smith, of the 

 University of Wisconsin, on "The Preservation of Fresh Water 

 Chlorophyceae." Among the preservatives recommended is 

 Amann's lacto-phenol-copper solution, and there are given also 

 the results of tests conducted by himself and others as to plas- 

 molysis and coloration. He also gives suggestions as to the 

 amounts which had produced better results with certain algae. 



Finding it necessary to make my collection as stable as possible 

 I made use of this solution, putting various algae into it, using 

 as suggested liberal amounts of the solution. I also made a few 

 tests using copper nitrate instead of copper chloride in the solu- 

 tion. The material in the solutions showed little change during 

 periods of one month, two months, or ten weeks. At the three- 

 months' examination, some of the algae showed signs of plas- 

 molysis. After nine months I find that the following results 

 have attended the use of these preservatives : 



Copper-Nitrate-lacto-phenol 



10 per cent, Vaucheria, color good, no plasmolysis 

 5 per cent, Vaucheria, color good, some plasmolysis 



10 per cent, Spirogyra, completely plasmolyzed and browned 

 5 per cent, Spirogyra, completely plasmolyzed and browned 



10 per cent, Telraspora, kept well in every case 

 5 per cent, Telraspora, kept well in every case 



