76 Composition of the oil from Bir Bahotc. { March, 1905, 
characteristic manner of cholesterol. The crystals were thin 
plates, but their shape was quite irregular. cr for cholesterol 
by the colour reactions the results were as fo 
op d 
modification of the above test in which the cholesterol is first 
of acetic anhydride and one drop of sulphuric acid. Salkowski’s =: 
oO 
teristic of cholesterol. A minute quantity was dissolved in 2 c.c. 
of chloroform and an equal volume of concentrated sulphuric 
added to it and the mixture shaken. On separating, the chloro- 
ioe layer was coloured red, and on standing changed to purple 
on the following day when the lower layer had a decided green 
fiuoresence. It should be stated that the ar as matter 
used in the above tests was quite free from colou 
: These reactions and the melting point of os alcohols obtained 
argent the presence of cholesterol, and or ssibly of a fatty alcohol 
high molecular weight. The amount of unsaponifiable matter 
available was too small for further investigation 
e Fatty acids—The soap freed from unsaponifiable matter 
was incited with dilute hydrochloric acid, and heated to liberate 
the fatty acids. These were thoroughly washed a dried in @ 
steam-oven hey were still tinted red, but not enough so to 
render impossible the use of phenol-phthalein as an indicator. 
or the mixed fatty acids the following values were obtained :— 
Saponitication value tive aa 489 
nce mean mol. wt. aes es 282) 
Jodine value ae ive 
= point .. 31°-32° 
acids obtained as Brit were treated i 
ing points were 54°. 7, 53°. 4 and 53°. 6, iF cciticaly. They ee 
ee very nearly pure, ‘but not quite, an nd the presence of myristic 
with a small quantity of palmitic or stearic acids was 
indiate. The three fractions were mixed and put on one 
si : 
The filtrate from the alcohol sp. gr. ‘911 were evaporated down 
to dryness and saponified with an alcoholic solution of potassium 
hydrate. The sel was exactly neutralized with acetic acid and 
