Vol. I, No. 4.] Colouring Principle of Nyctanthes Arbor-tristis. 103 
LN. 8.] 
colour was produced at the junction of the two liquids. This soon 
darkened and disappeared. 
en the infusion was allowed to stand for a few days alone, 
a reddish-brown deposit settled. If a little hydrochloric acid was 
added to the infusion, a reddish flocculent precipitate settled after 
about twelve hours. A similar precipitate was obtained by 
heating the infusion with basic lead acetate, decomposing the 
tained was always very small, but attempts to get more from the 
solution by heating on the water-bath with dilute hydrochloric acid 
resulted in the deposition of a black amorphous substance. When 
substance, which reduced Fehling’s solution on boiling. 
two grains of the red colouring matter were collected, 
acetate, and ether, but it could be made to crystallize from none 
of these, It also dissolved readily in alkalis and alkaline carbon- 
ates, and in a solution of borax. t was sparingly soluble in 
chloroform and carbon bisulphide, insoluble in benzene and cold 
pe very slightly soluble in hot water, and soluble in acetic 
acid. 
Alcoholic extract.—Owing to the apparent high solubility of the 
colouring matter in alcohol, some of the flowers were extracted In a 
Soxhlet apparatus with alcohol (sp. gr. ‘810) till they were colour- 
less, and the hot alcohol was then allowed to cool. On cooling, 
bunches. of needle-like crystals had settled all over the flask. 
Analysis gave :— 
Carbon =39°62 
Hydrogen. = 7:96 
i =39°57 
Oho, requires { ee 77 
The crystals melted at 166" ; 
Mannitol melts at 168°. 
When the filtrate from the mannitol was slightly evaporated 
furt i d, but a small quantity 
