DEVELOPMENT OF SPERMATOBIUM. I5 



1. Budding proper of the caryoplasm, by means of 

 fine, thread-like elongations from the nucleus proper. 



2. Formation of caryoplasmic nodes at certain at first 

 irregular, later on at regular intervals. 



3. Growth of these nodules by attraction and contrac- 

 tion of the outlying caryoplasmic threads. 



4. Final division of these secondary or micronuclei 

 by caryokinesis and their moving apart, forming the final 

 nuclei preparatory to the formations of the pseudonavi- 

 cella spores. 



5. To this may be added the division or disintegration 

 of the nucleolus, which takes place later on, and which 

 does not appear to be of importance in the formation of 

 the micronuclei. In some instances the nucleolus remains 

 intact for some time after the division and redistribution 

 of the caryoplasm. 



The first indication of a division of the macronucleus 

 is seen in the unequal distribution of the caryoplasm 

 Avithin the caryotheca. The latter at the same time as- 

 sumes an irregular outline and soon disappears entirely 

 (fig. 7a and b). But even before the caryotheca has 

 vanished, the caryoplasm has penetrated its walls and ac- 

 cumulated outside of, but adjacent to the latter. From 

 these agglomerations caryoplasmic filaments are seen ex- 

 tending irregularly in all directions (figs. 7 and 8) . These 

 caryoplasmic filaments when properly stained may be 

 found extending all through the cytospheres, winding 

 their way between them. At certain intervals there ap- 

 pear thicker nodes on the filaments and from these nodes 

 other filaments radiate in various directions. At last a 

 stage in radiation has arrived when nodes are found 

 at fairly regular intervals throughout the cytosome (fig. 

 9« and h). At this stage there frequently or nearly al- 

 ways appear one or more darker staining bodies in the 



