DEVELOPMENT OF SPERMATOBIUM. II 



possess a nucleolus, of distinct form and appearance, 

 which I will describe later on. In its earliest stage the 

 nucleus is clear, globular, surrounded by a circular cary- 

 otheca of considerable thickness. As, however, I have 

 frequently found the outline rosette-like (figs. 41c; T-b), 

 I believe that it possesses an amoeboid movement, al- 

 ready in the very early stage, when the protozoan is 

 3^et confined to the spermogonium of the host (fig. '^b). 

 In most of the intercellular Spermatobia the caryo- 

 theca was wavy, slightly folded, showing signs of hav- 

 ing altered its shape (fig. 41c and d). Some, however, 

 possessed the regular circular outline. The caryotheca 

 always stains readily but not deeply with the orange G. 

 Of the contents of the caryosome — disregarding the nu- 

 cleolus for the present — I could sometimes distinguish 

 two different substances: one protoplasmatic, by far the 

 most abundant, and also a darker staining, more regularly 

 grained part, probably the chromosomes. How far these 

 respective substances in the resting nucleus correspond 

 with the chromosomes and microcaryosomes, etc., of 

 higher nuclei I am unable to say, as they are not well 

 differentiated until in the latter stages of sporulation, where 

 chromosomes and filaments may be distinctly recognized. 

 We may distinguish several distinct stages of nuclear de 

 velopment, each one of which presents some characteris- 

 tics of importance : 



1. Resting macromicleus, with perfect caryotheca, dif- 

 fuse caryoplasma, single large nucleolus with several in- 

 tranucleolar bodies. 



2. Amoeboid micl ens, or the first stage of sporulation 

 in which the former resting macronucleus divides itself in 

 numerous micronuclei by an apparently amoeboid bud- 

 ding or diffusion of the caryoplasm in among the cyto- 

 spheres. The amitotic stage. 



