DEVELOPMENT OF SPERMATOBIUM. 3 



Habitat. In my late paper on Eclipidrilus I have re- 

 ferred to the occurrence of this annelid in two separate 

 localities at different elevations. One is a cluster of 

 springs on the south slope of the middle fork of King's 

 River in California, at an altitude of about ii,ooo feet. 

 The water in these springs is very cool — icy, in fact — 

 very clear, transparent, without apparent trace of sus- 

 pended sediment. The bottom is very sandy, here and 

 there covered with water mosses. The hosts of Sperm- 

 atobium live in the sand or fine sediment among the roots 

 of the moss, etc. 



In another locality, the Three Spring Meadow on the 

 north fork of King's River, this protozoa was not found, 

 though Eclipidrilus is common there, too. The water in 

 these springs is less pure with more sediment, and the 

 Eclipidrili were found crawling in decayed wood, etc. 

 The altitude was only about 7,000 to 8,000 feet. I refer 

 thus in detail to these localities, because other protozoa 

 Hcemagi'egai'ina nasuta were found to infest in countless 

 numbers the same hosts, from the higher altitude and 

 the purer and cooler water, while in those from the lower 

 locality and the less pure water they were totally absent — 

 that is, the Hcsniagi'egarina nasttta as well as Spermato- 

 biiini eclipidrili. 



Phoenicodi'iliis taste occurs in the mountains of the Cape 

 Region of Baja California, at an altitude of 4,000 feet, 

 and lower down to the coast, about fifty to sixty miles 

 north of Cape San Lucas. 



METHOD OF INVESTIGATION. 



The Eclipidrili were in rather poor state of preserva- 

 tion, having been hardened and kept in alcohol. The 

 Phoenicodrili had been carefully fixed in a solution of cor- 

 rosive sublimate and afterwards hardened, some in abso- 



