APPENDIX B 

 DEVELOPMENT OF SAMPLING PLAN 



1 . Ocean Beach Sampling . 



Initially, in August 1975, six sampling sites were established on 

 each of the three ocean beach transects. Sampling sites on these tran- 

 sects were placed with respect to MSL. Site 1 was 7.6 meters 

 (horizontal distance) shoreward of the MSL point (zero point) , and 

 sites 2 to 6 were 7.6, 15.2, 30.4, 45.6, and 60.8 meters seaward from 

 the MSL point. Three samples, each consisting of two cores, were taken 

 at each site (total area sampled 1.15 x 10"^ square meters by 10 centi- 

 meters deep). Samples were placed in prelabeled plastic bags, stored 

 at 1° to 4° Celsius, and returned to the laboratory for extraction. 

 Samples were sieved, and the organisms retained on the 0.6- or 0.4- 

 millimeter sieves were collected. Organisms were preserved in 

 4-percent Formalin for later identification. 



Salinity and temperature readings were taken at all sites, and an 

 additional core sample was taken at odd-numbered sites on each transect 

 for analysis of grain size and for determination of organic and 

 carbonate content. The results of the initial sampling are discussed 

 in Appendix C. 



In the October-November and subsequent samplings the zero point on 

 the transects was moved from MSL to the landward margin of the swash 

 zone. Site 1 was abandoned and four new sites (sites 1, 3, 4, and 6, 

 respectively) were established at 3.3, 10.6, 13.6, and 22.7 meters 

 seaward of the zero point. An improved technique was used to extract 

 the organisms from the core samples and core samples for grain size 

 analysis were taken at all sites rather than every other site. In the 

 March-April and subsequent samplings the number of samples per site 

 was increased from three to four and sampling at sites 4 and 6 was 

 eliminated. These changes and the resulting final plan are discussed 

 in detail in the text in Section II, 3. 



2. Sound Beach Sampling. 



Initially in July 1975, six sampling sites on each of the three 

 sound beach transects were placed with reference to the sound margin 

 (zero point) at 15.2, 38.0, 51.8, 61.0, 68.6, and 76.2 meters from the 

 shoreline. The procedures for sampling and sample extraction were the 

 same as described for the August 1975 ocean beach samplings. 



Salinity and temperature readings were taken at all sites, and an 

 additional core sample was taken at odd-numbered sites on each transect 

 for analysis of grain size and for determination of organic and 

 carbonate content. The results of the initial sampling are discussed 



in Appendix D. 



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