508 U.S. NATIONAL MUSEUM BULLETIN 271 



Pereopods and gnathopods are removed to one side of the dish 

 until all seven legs have been collected. Antennae 1 and 2 are dissected 

 at their bases (right side only). Care in removal of antenna 2 at its 

 juncture is needed because it often breaks easily at joint 2 or 3. 



The seven coxae-legs and two antennae are removed in a group 

 from the dish of alcohol to the flat sUde with the largest drop of glyc- 

 erine. When placed in the glycerine the parts will disperse the drop, 

 but a light breath of air will accelerate evaporation of the alcohol 

 and the amalgamation of the puddle. The legs must be fully immersed 

 in the glycerine to prevent drying and uptake of air bubbles. Do 

 not put on the cover shp. 



The right uropods 1, 2, and 3, both lobes of the telson, and one 

 member of each pair of the pleopods are removed and placed on two 

 of the flat slides wdth tiny drops of glycerine; the parts are manip- 

 ulated, while the glycerine puddles coalesce, and arranged so that 

 their respective dorsal (uropods) and anterior (pleopods) sides are 

 up. A clean cover slip, gripped in the forceps, is lowered horizontally 

 over the glycerine until it can be dropped smartly onto the puddle 

 without engaging air bubbles. Glycerine is to be applied sparingly 

 so as to prevent excessive sUding of the cover slip. If the perimeter 

 of the cover sUp lacks glycerine it may be added later by placing a 

 small drop at the edge. 



Before removing mouthparts determine whether they are grouped 

 in a coniform or quadratiform bundle from lateral view. 



Mouthparts are removed from the head, again with the amphipod 

 head pointing away from the observer so that motion to the right 

 A\dth the forceps can be used to snap off the mouthparts. The maxil- 

 lipeds, which are the most posterior mouthparts, cover all the other 

 mouthparts and must be removed at their base first; both maxilUpeds 

 A\dll come off together. More anteriorly, a pair of bilobed second 

 maxillae is to be removed and then the first maxillae, each of which 

 appears to have three lobes (inner lobe, outer lobe, and palp but in 

 a few genera lacking a palp). The inner lobes are difficult to remove in 

 connection with the outer unless special care is taken and caution 

 must be exercised not to damage the lower lip. Mandibles are removed 

 next; they are usually brittle and easily broken; they are most easily 

 removed by rotating them to ascertain the basal muscular attach- 

 ment and snipping this mth forceps. Sclerotic connections to upper 

 and lower fips also must be broken to avoid their damage. Usually 

 each mandible will have a palp. After maxilla 1 and the mandibles 

 are removed, a lower lip and an vipper Hp will remain; the lower lip 

 is extensive and for removal must be grabbed deeply in its muscular 

 and tendon attachments without separating the inner and outer lobes. 

 After practice one may desire to remove lower lips before dissecting 



