[33] THE PRESERVATION OF MARINE ANIMALS HOVEY. 



Aphroditidee certain Polynoinse, Polyodontes maxillosuSj of the Eunici- 

 dfe all the group of the Eunicinaj. Some of these, like Dioptara, are 

 best fixed by narcotizing them in alcoholized sea water. 



AlciopidfB are very well prepared by letting them die in the sulphate 

 of copper and sublimate mixture. They should remain in the solution 

 not to exceed five minutes, and then be washed thoroughly in fresh 

 water before they are placed in alcohol. 



Tomopteridse are preserved in the way just described, or with cold 

 saturated sublimate. Eemove the last traces of sublimate with iodine. 



CKUSTACEA. 



The marine Oladocera {Podon, JEvadne) are killed with saturated 

 sublimate, or with a few drops of osmic acid of 1 per cent in the sea 

 water containing them, removing them when they begin to turn brown. 

 Wash and put into 70 per cent alcohol. 



Ostracoda are put at once into 70 per cent alcohol. 



Copepoda. — The free forms are killed in a saturated solution of sub- 

 limate in sea water, where they are allowed to stay from five to ten 

 minutes. The parasitic forms may be killed in the same way or be put 

 at once into weak alcohol. 



Girripedia. — To prepare Lepas, Gonehoderma, etc., with the cirrhi dis- 

 tended, let them die in alcohol of 35 per cent. If the cirrhi of certain 

 species contract they can easily be drawn out again by means of forceps. 



Balanus and similar forms are immersed directly in alcohol of 70 per 

 cent, taking care to change the liquid soon. 



BMzocephala {Sacculina, Peltogaster, etc.) are placed for fifteen min- 

 utes in a mixture of 90 per cent alcohol and sublimate in equal parts, 

 and then transferred to 70 per cent alcohol. 



Amphipoda. — All the Lsemodipodes, Crevettines and Iperines were 

 formerly prepared by putting them at once into alcohol of 70 per cent, 

 but now they are put into Perenyi's solution for fifteen minutes before 

 they are immersed in the alcohol. The transparent forms of the last 

 division {Phronima, etc.) are killed in sublimate. 



Isopoda. — These are put at once into 70 j)er cent alcohol, with' the 

 exception of the Bopy rides and Bntoniscides, which are first placed in 

 a mixture of 90 per cent alcohol and saturated sublimate in equal parts 

 (like the Ehizocephala) or in saturated sublimate alone. 



Giimacea and Stomatopoda go directly into alcohol, though the trans- 

 parent larval forms of the latter are first put into saturated sublimate 

 for a few minutes. 



ScMzopoda go at once into alcohol or they may be treated first with 

 saturated sublimate. 



To avoid the breaking of the appendages of the Becapoda, these 

 forms are allowed to die in fresh water before they are put into alcohol. 

 They remain in fresh water only as long as is necessary, otherwise the 

 membranous appendages are injured. * 



2138— No. 39, Pt. M 3 



