sampling plan, it is necessary to have some measure of the gross 

 "patchiness" of the populations. Patchiness refers to the aggregation 

 or clumped distribution of individuals in which distribution is not ran- 

 dom and the variance is significantly greater than the mean value. Sam- 

 pling can be stratified by locating stations at different depths, but 

 without further preliminary sampling it is not known whether the magni- 

 tude of spatial variation of the endofauna warrants the further strati- 

 fication of sampling within a depth contour. 



To determine the gross patchiness of the endofauna, sets of replicate 

 samples should be taken randomly within depths from progressively larger 

 areas. If any of the major parameters change significantly from one 

 depth to the next, it is necessary to adjust the sampling plan (e.g., 

 stratify) to produce a more reliable estimate of the population. 



V. SAMPLING METHODS 

 1. Establishing Sampling Transects . 



After the sampling methods and number of replicate samples needed 

 are determined, transects can be established and marked by inserting 

 stakes or screw anchors at the upper limit of the transects (Fig. 4). 



VI 



-to5-in Diometer. ^P|3 



r i 



3 to 48 in 



Figure 4. Screw anchor, 



17 



