14 



THE VENOMS OF CERTAIN THANATOPHIDE^E. 



we find that, while they have very close resemblances, as they naturally should since 

 they are so intimately related, they are very readily distinguished from each other. 

 The properties of all globulins are so readily affected by even the simplest manipu- 

 lations that it is likely that mere precipitation may affect them in regard to their 

 solubility, while drying may completely destroy this property. Having these facts 

 in mind, it seems almost a necessity that the processes through which we put these 

 globulins, in order to get them isolated in a pure state, has more or less modified 

 their chemical, and possibly their physiological properties. 



The tests made with these globulins were all made at different times, the one 

 globulin was examined one day, and another on another day, so that the reactions 

 given are not absolutely accurate as a matter of comparison, but only relative, since 

 the standard of solubility, which was of course an arbitrary one, was simply carried 

 in the mind throughout the examinations. We believe, however, that they are 

 practically correct. 



Keagent. 



Water-venom-globulin . 



Copper-venom-globulin. 



Dialysis- venom-globulin . 



Sodic chloride (10 p. e.) 



Soluble 



Insoluble 



Slightly soluble. 



Carbonic acid 



Soluble 



Insoluble 



Soluble. 



Sodic carbonate 



( Very soluble ; not 

 [precipitated by C0 2 



Very soluble ; pre-) 

 cipitated by C0 2 j 



Very soluble. 



Hydrochloric acid (0.4 p. c.) 



Very soluble 



Very soluble 



Very soluble. 



Metaphosphoric acid 



Insoluble 



Insoluble 



(Insoluble; rendered 

 (of a yellowish tint. 



Orthophosphoric acid 



Soluble 



Very soluble 



Very soluble. 



Sodic metaphosphate 



Insoluble 



Insoluble 



Very soluble. 



Sodic orthophosphate 



Very soluble 



Less soluble 



Still less soluble. 



Potassic sulphate 



Very soluble 



Insoluble 



Insoluble. 



Calcic chloride 



Very soluble 



Less soluble 



Less soluble. 



Acetic acid (5 per cent.) 



Very soluble 



Soluble 



Very soluble. 



Acetic acid (glacial) 



Very soluble 



Soluble 



Very soluble. 



The venom of the Moccasin (Ancistrodon piscivorus) was subjected to an analysis 

 similar to that of the Crotalus, the isolated proteids giving the following reactions: — 



Water-venom-globulin. 



Decided reactions with the usual proteid color tests. 



Boiling — clears the mixture without the apparent formation of any coagula. 

 Sodic chloride (0.75 per cent.) — insoluble. 



(10 " ) — somewhat soluble, solution not absolutely clear ; boiling clears 



absolutely without the formation of coagula. 

 (crystals) — somewhat soluble ; solution not precipitated by carbonic acid. 

 Carbonic acid — insoluble. 

 Sodic carbonate — soluble, forming slightly turbid solution ; boiling clears the solution without 



giving coagula; the addition of crystals of sodic chloride to 



the hot boiled solution causes a precipitate, this precipitate 



being coagulated by boiling. 

 Hydrochloric acid (0.4 per cent.) — somewhat soluble. 



(5 " ) — soluble. 

 Metaphosphoric acid — insoluble. 

 Orthophosphoric acid — soluble. 



Sodic metaphosphate — slightly soluble; solution rendered clearer by boiling. 

 Sodic orthophosphate — soluble; solution rendered absolutely clear by boiling. 



