16 



THE VENOMS OF CERTAIN THANATOPHIDE.E. 



9. Precipitated by mercuric chloride. 



10. Precipitated by absolute alcohol. , 



11. Gives a faint reddish tinge with a strong solution of potassium hydrate, and a trace of cuprie 

 sulphate. 



12. Not precipitated by strong acetic acid (glacial). 



13. Precipitated by very dilute acetic acid ; precipitate being redissolved by further addition of acid. 

 14 Full reaction with Adamkiewicz's test for proteids. 



15. Precipitated by adding a large quantity of sodium chloride, the precipitate being redissolved 

 on the addition of a large quantity of glacial acetic acid. 



16. Precipitated by mercuric nitrate. 



17. Precipitated by absolute alcohol; precipitate being apparently redissolved on the addition of 

 water. 



18. Precipitated by saturation with potassium hydrate; precipitate being redissolved by the addi- 

 tion of nitric acid, with the formation of a decidedly yellow solution (xantho-proteic) which becomes 

 decolorized by addition of acid. 



19. Precipitated by potassium ferrocyanide in the presence of weak acetic acid. 



Venom-peptone by dialysis gives identical reactions. 



For convenience of comparison we append here in parallel columns the principal 

 reactions of the Moccasin globulins, remembering in this connection the difference 

 in the properties manifest in their methods of preparation. 



Reagent. 



Water-venom-globulin. 



Copper- venom-globulin. 



Dialysis-venom-globulin. 





f Clears almost abso- 





Clears some. 



Boiling 



\ lately 



Clears some 



Sodic chloride (10 per cent.) 



Somewhat soluble 



Insoluble 



Somewhat soluble. 



Carbonic acid 



Insoluble 



Somewhat soluble 



Very soluble. 



Sodic carbonate 



Soluble 



Very soluble 



Very soluble. 



Hydrochloric acid (0.4 p. c.) 



Somewhat soluble 



Very soluble 



Very soluble. 



3Ietaphosphoric acid 



Insoluble 



Insoluble 



Slightly soluble. 



Orthophosphoric acid 



Soluble 



Very soluble 



Very soluble. 



Sodic metaphosphate 



Somewhat soluble 



Insoluble 



Insoluble. 



Sodic orthophosphate 



Soluble 



Less soluble 



Soluble. 



Potassic sulphate 



Soluble 



Insoluble 



Slightly soluble 



Calcic chloride 



Soluble 



Insoluble 



Very soluble. 



Acetic acid (5 per cent.) 



Insoluble 



Soluble 



Soluble. 



Acetic acid (strong) 



Insoluble 



Soluble 



Soluble. 



For reactions of the peptones of the various venoms see p. 19. 



Cobra Venom. — We have been able to isolate in Cobra venom only two proteids, 

 and these correspond in their characters to the two types of proteids found in 

 the venoms of the Crotalus and Ancistrodon. In other words, we have isolated a 

 globulin and a peptone-like principle. The globulin we are able to precipitate 

 completely by the addition of a proper amount of distilled water, after which the 

 solution gives no coagulum by boiling. There is then left in solution a proteid, 

 which evidently belongs to the peptones, although giving some extraordinary 

 reactions. 



The venom-globulin thus isolated and purified, as in the preparation of the 

 globulins previously mentioned, possesses the peculiar properties of the globulin 

 family, and, in accordance with our nomenclature, since it is entirely precipitated 

 by the addition of distilled water, is a water-venom-globulin. 



