18 



THE VENOMS OF CERTAIN T H A N A T P H I D E^E. 



The dialysate was perfectly clear and 



by dialysing venom for forty-eight hours, 

 neutral in reaction : — 



Boiling — no result until after a few moments, when it becomes cloudy, the cloudiness increasing 



as boiling continues ; strong nitric acid dissolves the precipitate. 

 Color reactions for proteids — the xantho-proteic, Millon and Biuret reactions are all obtained. 

 Ferric chloride — no effect. 

 Gupric sulphate — no effect. 

 Mercuric chloride — no effect. 

 Mercuric nitrate — decided precipitate. 

 Absolute alcohol — no precipitate. 



Potassic ferrocyanide -|- weak acetic acid — precipitate. 

 Nitric acid (strong) — no precipitate. 

 Hydrochloric acid (strong) — no precipitate. 

 Acetic acid (strong) — no precipitate. 



Sodic chloride (saturation) — precipitate; acetic acid, large quantity, dissolves. 

 Potassic hydrate to saturation — precipitate. 

 Tannic acid — decided precipitate. 

 Basic acetate of lead — decided precipitate. 



Several very remarkable facts are the coagulation by prolonged boiling and the 

 non-precipitation by mercuric chloride and absolute alcohol. Since this peptone 

 is precipitated by weak acetic acid in the presence of potassic ferrocyanide it has 

 a slight resemblance to Meissner's A peptone, although materially differing, as 

 ■some of the above reactions show, from any other described body of this class. 



As a matter of some interest, it is desirable to know if similar globulins in 

 different venoms are identical in their chemical nature, or whether they give any 

 reactions which may distinguish them. We have accordingly, as in previous cases, 

 placed the reactions of the corresponding globulins side by side. 



I. Water-venom-glohitlin. 



Reagent. 



Crotalus horridus. 



Ancistrodon piscivoris. 



Cobra. 



Boiling 



Coagulates 



Apparently dissolves 



Coagulates. 



Sodic chloride (10 per cent.) 



Soluble 



Somewhat soluble 



Soluble. 



Carbonic acid 



Soluble 



Insoluble 



Insoluble. 



Sodic carbonate 



Soluble 



Soluble . 



Soluble. 



Hydrochloric acid (0.4 p. c.) 



Soluble 



Somewhat soluble 



Soluble. 



Metaphosphoric acid 



Insoluble 



Insoluble 



Insoluble. 



Orthophosphoric acid 



Soluble 



Soluble 



Soluble. 



Sodic metaphosphaie 



Insoluble 



Somewhat soluble 



Insoluble. 



Sodic orthophosphate 



Very soluble 



Soluble 



Somewhat soluble. 



Potassic sulphate 



Very soluble 



Soluble 



Somewhat soluble. 



Calcic chloride 



Very soluble 



Soluble 



Soluble. 



Acetic acid (5 per cent.) 



Soluble 



Insoluble 



Soluble. 



Acetic acid (strong) 



Soluble 



Insoluble 



Soluble. 



