40 SPOROZOA. 



in a larger tube (the ordinary bacteriological potato -tube) 

 provided with a constriction on which the smaller tube may 

 rest. In the portion of the outer tube below the constriction 

 put some solution of pyrogallic acid and add 2 or 3 c.c. of 

 •a 10 per cent, solution of sodium hydrate immediately before 

 introducing the smaller tube. Cork the larger tube tightly 

 with a rubber cork. The pyrogallic acid and sodium hydrate 

 absorb the oxygen, and the culture thus takes place in an 

 oxygen-free atmosphere. 



Sinton's Method. — A specially constructed tube about 20 cm. 

 in length is employed (fig. 2). To prepare this apparatus, 

 take a tube of 0-4 to 0-5 cm. bore. Draw out one end as in 

 an ordinary pipette and, shpping a narrow metal tube over 

 the thin drawn-out portion while it is still soft, press upwards 

 to produce a dilated bulb with its lower surface flattened (B) 

 a,nd with the thin drawn-out tube arising from it (A). Now 

 Jieat the tube a short distance, about 1 cm., above the flattened 

 surface and draw out till it forms a tube about 6 to 8 cm. 

 long and 0-2 cm. wide (C). At the upper end of this narrow 

 tube make a sHght constriction (D), and about 0-4 to 0-5 cm. 

 ■above it make another (E). Drop three glass beads (F) 

 into the upper part of the tube (G), which is allowed to remain 

 wider in diameter, and then draw out and bend as in a Wright's 

 capsule (H). Keep the upper and lower drawn-out ends sealed 

 and sterihze the whole tube. 



To make the culture, open the tube at both ends, and bend 

 the upper capillary portion at right angles to the rest, so that 

 the apparatus may lie on the table with the open upper end 

 pointing upwards. Insert the upper end into an already 

 prepared Wright's capsule containing ascitic or hydrocoele 

 fluid, to which the requisite quantity of dextrose has been added, 

 and allow the fluid to enter till the upper section is about a third 

 or half full. Prick the carefully steriHzed finger of a malarial 

 patient, and allow five to ten drops of blood to run into 

 the fluid in the tube. Now gently heat the dilated part of the 

 lower end of the tube and seal off the narrow part below it. 

 The air in the dilated part will cool and the blood-mixture will 

 be drawn further into the tube. Now seal ofl" the upper end 

 also. Defibrinate the mixture by shaking the glass beads, 

 and when this has been completed, swing round the tube 

 rapidly so as to drive the defibrinated blood-mixture through 

 the constriction into the lowermost part of the tube, so that 

 it fills the dilated part and the narrow section above it. 

 Heat the tube at the constriction above the column of fluid 

 and seal ofl". The red corpusc]es will settle to the flat bottom. 

 Incubate the tube in a vertical position at a temperature 

 of 35° to 38° C. In order to examine, open the tube, withdraw 

 by a pipette the cells from the lower end, and seal again. 



