HEPATOZOON. 147 



the gametocytes closely resemble H. canis and H. muris. 

 Nothing is known of the sexual cycle in the Invertebrate. 



Habitat. — Blood and smears from the lung of an Indian 

 eagle (not identified) : Punjab, Kasauli. 



85, Hepatozoon canis (James). (Fig. 67.) 



■\Leucocytozoon canis, James, 1905, pp. 1-12, 1 pi. ; Bentley, 1905, 

 p. 988; Christophers, 1906, pp. 1-16, 1 pi.; 1907, pp. 1-12, 1 pi. 

 'fLeucocytozoon bentleyi, James, 1905 a, p. 1361. 

 Leucocytogregarina rotundata, Patton, 1910, p. 280. 

 Heemogregarina canis adusti, Nuttal, 1910, p. 108. 

 Hasmogregarina canis, Wenyon, 1911, pp. 324-32, text-figs. 4-8, 



pi. xvi, figs. 1—17. 

 Leucocytozoon canis, Christophers, 1912, pp. 37-4:4:, 1 diagram, 



text-figs. 4-8. 

 Hepatozoon canis, Minchin, 1912, p. 377. 

 Heemogregarina canis, Castellani & Chalmers, 1919, p. 483. 

 ^Hepatozoon canis, Rau, 1925, p. 293. 

 \Hsemogregarina canis, Rau, 1926, p. 244. 

 Hepatozoon canis, Wenyon. 1926, pp. 1091, 1092, fig. 455 ; pi. xix, 

 figs. 3, 4 ; pp. 1355-7 ; Reichenow, 1929, pp. 921, 922, figs. 885, 

 886. 



Schizogony occurs in the spleen, bone-marrow or liver. 

 Schizonts of several types ; some produce a small number 

 (generally three) of large merozoites, which become schizonts 

 again, whilst others produce a large number of small merozoites. 

 These latter enter the mononuclear or polymorphonuclear 

 leucocytes and become gametocytes. Free vermicules found 

 in the gut of the infected ticks. 



The gametocytes undergo development in the common 

 dog-tick, Ehipicephalus sanguineus Koch, in the tissues of 

 which are eventually produced very large oocysts, containing 

 from 30 to 50 sporocysts, each containing about 16 sporozoites 

 and a residual body. After the sporocysts are fully developed, 

 the oocyst appears to break up and sporozoites are found 

 scattered among the tissues of the tick. Infection of the dog is 

 probably produced by the ticks being eaten. 



Dimensions. — Gametocytes 8-10 ^u, by 4-5 /x, nucleus 2-3 ft; 

 oocysts up to 100 ju, ; sporocysts 15-16 /a in length ; sporozoites 

 14-15 /A in length. 



Remarks. — Rau (1925) infected dogs by injection of spleen 

 material containing schizonts, as also by the injection of the 

 tissues of infected ticks. Parasites appeared in the blood 

 two or three weeks after inoculation and infection progressed 

 in intensity, bringing about the death of some of the animals. 

 Wenyon, however, doubts if other causes of death were 

 excluded. 



Habitat. — Internal organs and leucocytes of the Indian wild 

 dog, Gyon duhhunensis (Sykes) : Assam, BorjuHe, Gauhati, 



l2 



