ADDENDA. 389 



Genus ANAPLASMA Theiler, 1910. 

 (Pages 325-6.) 

 319. Anaplasma marginale Theiler. 



" Marginal points," Smith & Kilborne, 1893. 



Anaplasma marginale, Theiler, 1910, pp. 135—7 ; Wenyon, 1926^ 

 pp. 1053-6. 

 "f Anaplasma sp.. Ware, 1932, pp. 31—2. 

 ^Anaplasma m,arginale, Ware, 1938, p. 212. 



Spherical granule, varying in size from 0-1 to 0-5 /x ; staining 

 bright red with Romanowsky's stain, and situated near the 

 margin of the red blood-corpuscle. 



Remarks. — ^Anaplasmata have been encountered in the 

 blood of hill bulls at Muktesar and the animals were regarded 

 as " carriers." Dias and Aragao (1914) claimed to have 

 brought about the production of these bodies in cattle by the 

 simple injection of certain poisonous substances such as trypan 

 blue. Du Toit (1928) expressed the view that the production 

 of true Anaplasmata in cattle by repeated injection of trypan 

 blue can only be brought about when the cattle are " carriers," 

 the injections causing a breakdown in their immunity, with 

 the appearance of parasites in their blood. Ware (1932) 

 reported the appearance of these parasites in hill bulls after 

 a series of trypan blue injections. 



Habitat. — Blood of cattle : United Provinces, Muktesar ; 

 Central Provinces. 



Genus BERTARELLIA Carini, 1930. 



(Pages 326-7.) 



320 Bertarellia carinii de Meja-elles. 



Bertarellia carinii, de Meyrelles, 1938, pp. 49-53, figs. 1 & 2. 



Usually roundish or elliptical, rarely oval, pyriform or ring- 

 like bodies stained violet with Leishman's or May-Grunwald 

 Giemsa's stain. Each such chromatic point or anaplasmoid 

 body is surrounded by a clear halo, often difficult to dis- 

 tinguish, and roundish or elliptical in form. The parasite 

 may be central, polar or peripheral in position in the red 

 blood-cells, or may even be found free in the plasma. The 

 red cell may contain more than one parasite, and the halo- 

 like portion may contain two or even three chromatic granules, 

 suggesting binary division. 



Remarks. — The organism differs from B. calotis, as the 

 cytoplasm is never stained blue as in that species. It is, 

 however, identical with the parasite found in blood-films 

 from two Brazihan tortoises. 



Habitat. — Blood of the Indian tortoise, Lissemys punctata 

 granosa Smith : Portuguese India, Nova Goa. 



