CONTENTS 



Page 



INTRODUCTION 1 



METHODS AND MATERIALS 1 



Ocean Exposure 1 



Bacterial Cultures 1 



Bacterial Counts 2 



EXPERIMENTAL RESULTS 2 



Effect of Sea Salts on the Survival of E^ coli. 2 



Effect of Ocean Depth on Survival of E^ coli. 3 



Effect of Sunlight on the Survival of E^ coli 4 



Sunlight Effect on _E. coli in Glass and in 



Polyethylene 6 



Effect of Sunlight on Seawater 6 



DISCUSSION OF RESULTS 8 



CONCLUSIONS 10 



ACKNOWLEDGMENTS 11 



REFERENCES 19 



LIST OF ILLUSTRATIONS 



Figure 1. Diagram of ocean exposure station 12 



Figure 2. Lowering steel drums from which sample line will 



be suspended in the ocean 13 



Figure 3. Weight for holding sample line taut in the ocean 13 



Figure 4. Winch for lifting and dropping sample line and 



anchor in the ocean 14 



Figure 5. Serial dilution of water samples for bacterial 



counts - . 14 



