The third set of samples taken during the fall of 1977 was washed in 
filtered seawater and decanted as before. Cover slips were used as before. 
After the last of the cover slips were processed, 10 grams of each sample was 
placed in 50 millimeters of acidified distilled water. Each sample was then 
sonicated using an E/MC Corporation Sonicator (model No. BP-1) according to 
the procedure described by Hickman (1969). Both the sand and the elutriate 
from each sample were examined. The sand was examined as before, and the elu- 
triate was added drop by drop to individual cover slips on a hotplate and 
allowed to dry. The cover slips were then cleared and mounted as before, and 
observed under magnification. 
The fourth and final samples taken during the winter of 1978 were not 
washed as in sample sets 2 and 3. Cover slips were placed on the surface of 
each sample, and again sand grains were examined. Additionally, a second set 
of cover slips was left in place for 2 weeks before processing. During this 
time, the samples were exposed to a 12/12 light-dark cycle with light provided 
Dy four 40-watt Gro-luxR fluorescent lamps. 
(2) Intertidal Macrobenthos Methods. Identifications were made with 
the aid of several taxonomic references (Pettibone, 1963; Williams, 1965; 
Schultz, 1969; Gosner, 1971; Bousfield, 1973; Gardiner, 1975). Identification 
of the species in the genus Donaz was made by H. Porter (University of North 
Carolina, Institute of Marine Science, Morehead City, North Carolina, personal 
communication, 1977). 
Specimens were counted and measured to the nearest 0.1 millimeter (except 
worms, which were not measured). Donax spp. and Hmertta were divided into size 
classes according to length. For Donax spp., length was defined as the longest 
anterior-posterior distance. For EH. talpotda, length was measured from the tip 
of the rostrum to the posterior end of the carapace. 
(3) Migrating Consumer Methods. Gut contents were examined for all 
fish taken and identified to the extent possible, labeled, and preserved. AI1 
crabs taken were identified, using the monograph of Williams (1965), and 
measured (carapace width from point to point). 
b. Statistical Methods, The experimental design of this project allowed 
all parametric data (population and density estimates) to be analyzed using a 
nested analysis of variance. Several measurements of community structure were 
employed: 
Shannon-Weaver's Diversity Index (Pielou, 1966) 
s 
BS = 3 Py log 2. 
where P. is the proportion of individuals of the eh species. 
Species Richness Index (Pielou, 1969) 
Hmax = Log 5S 
where S is the number of species present. 
24 
