The nutrient medium used on which the bacteria produced the antibiotic 
substance had the following composition: 
Bacto-tryptose 4 gm 
Bacto-neopeptone 1 gm 
Yeast extract 0.5 gm 
Ferric phosphate 0.1 gm 
Bacto-agar 15 gm 
Seawater (aged) 1,000 ml 
pH adjusted to TL = 13 
The bacteria had the following morphological and cultural characteristics: 
Cell: Gram negative rod 
Orange-pigmented, 
A lony: , 
Soames circular, and flat 
Growth in nutrient medium 
made with distilled water: Tides Gi geri 
Growth in nutrient medium 
5 Excellent growth 
made with seawater: - g 
This indicates that the bacterium was a true marine specie, and not a transplanted 
terrestrial specie. 
Test Materials 
For evaluating deep-ocean biological effects on nonmetallic specimens, two 
racks (bio-racks) were attached to the STU. The bio-racks were made of 1/4-inch- 
thick mild steel, and were covered with a vinyl red lead primer paint. The racks held 
ten plastic rods, four tubes, one pipe, and one rubber tube, all 3 feet long (Figure 8). 
The sections of the 3-foot-long plastic rods, tubes, and pipe, and rubber tube 
were treated in different ways. One section of each specimen was roughened, a 
second section was wrapped in burlap (a coal tar was used to adhere the first 
layer of the burlap to the specimen), a third section was taped with a friction tape 
and plastic electrical tape, and the fourth section was left smooth. The various 
wrappings were to provide a favorable foothold for the attachment and growth of 
deep-ocean fouling and boring organisms. A large piece of untreated fir wood was 
fitted around each of the specimens to act as bait to attract and lead any marine boring 
animals into direct contact with the specimen materials. 
