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The Anatomy of Lottia gigantea &ray. 



By 

 « Walter K. Fisher. 



Leland Stanford Junior University, California. 



With plates 1 — 4 and 13 figures in text. 



Owing to the relatively large size of Lottia gigantea and the 

 accessibility of fresh material, the following paper was undertaken 

 primarily with the view of investigating the nervous system in some 

 detail. At the same time it was deemed advisable to work out the 

 rest of the anatomy, because for a long time many points in the 

 organization of the Acmaeidce have been subject to lively controversy. 

 It is hoped that the present contribution will aid in settling some of 

 these questions. 



The work has been carried on at the Leland Stanford Junior 

 University, and at the Hopkins Seaside Laboratory, Monterey Bay, 

 California. In this connection I wish to express my gratitude to 

 Dr. Harold Heath, who suggested to me the problem and who has 

 since been ever ready with encouragement and kindly advice. For 

 the facilities of the Hopkins Seaside Laboratory I am much indebted 

 to the directors, Dr. C. H. Gilbert and Dr. 0. P. Jenkins. I am 

 likewise under obligations to Dr. W. H. Dall for naming a small 

 collection of Acmaeidce from the coast, and to Mr. W. F. Allen for 

 several times obtaining material. 



Methods. For general dissection there is no better method 

 than to place animals, killed in fresh water, into 70 % alcohol and 

 then into 90. After they are thoroughly hardened they should be 

 returned to about 70 °/ permanently and allowed to remain a year 

 or two. The weak alcohol makes the tissues pliable and one can 

 accomplish more than with freshly hardened specimens. Small in- 

 dividuals for sectioning should be killed in either Vom Bath's fluid, 

 or ordinary alcohol. For the circulatory system I injected fresh 



Zool. Jahrb. XX. Abth. f. Morph. i 



