41 



contained controls for background adsorption to empty wells, 



125 

 inhibition of I-Con A binding by 30 minutes of preincu- 

 bation with Con A (100yg/ml) , specific ligand (a-methyl-D- 

 mannopyranoside (MDM) , 2mM or non-specific protein (BSA, 

 lOOug/ml) . 



Identical procedures to those described above were 



3 

 used for determination of binding of H-DM-ADGG-Con A con- 

 jugates. For these experiments the specific activity was 

 3.46 x 10 cpm/yg and additional controls for the interfer- 

 ence of ADGG with the binding were performed. 



