Figure 7. INHIBITION OF CALF THYMUS RNA POLYMERASE II 

 BY ADH-BSA, ADH AND a-AMANITIN 



Results are expressed in a Dixon plot of the reciprocal 

 of velocity expressed as ypmole H-UMP incorporated 

 per minute versus concentration of inhibitor for three 

 different substrate (UTP) concentrations: 0.004 mM (#), 

 0-008 mM (A) and 0.016 mM (■). Least squares analysis 

 was used to obtain the best fit for each line. For ADH 

 and ADH-BSA, the concentrations of a-amanitin were 

 determined from the diazo linkage extinction coefficient. 



