TABLE OF CONTENTS 



Page 



ACKNOWLEDGEMENTS ii 



ABSTRACT i V 



SECTION I INTRODUCTION 1 



Objectives 1 



Background 2 



Rationale 14 



SECTION II MATERIALS AND METHODS 17 



Modification of a-Amanitin for 



Conjugation to Proteins IV 



Synthesis of cc-Amanitin-Protein 



Conjugates 21 



Biochemical Characterization of 



Conjugates 28 



Interaction of Conjugates with 



Cultured Cells 32 



SECTION III RESULTS 42 



ADH-BSA Conjugates 42 



ADH-Con A Conjugates 68 



ADGG-BSA Conjugates 75 



Hippuric Acid-Con A Conjugates 85 



ADGG-Con A Conjugates 101 



SECTION IV DISCUSSION 132 



ADH-BSA Conjugates 132 



ADH-Con A Conjugates 135 



ADGG-BSA Conjugates 136 



Hippuric Acid-Con A Conjugates 137 



ADGG-Con A Conjugates 140 



REFERENCES 151 



BIOGRAPHICAL SKETCH 159 



ill 



