Figure 16. EFFECT OF PK AND EDC CONCENTRATIONS ON 

 14 C-HA-CON A CONJUGATION 



Reactions were carried out in 1.0 ml volumes of 0.1 M 

 sodium phosphate buffer containing 1.0 pmoles of 1 C- 

 HA (8.5 x 10 5 dpm/iimole) , 10 or 100 iimoles of EDC and 

 0.001 ymoles of Con A. Results are expressed as the 

 total activity of the TCA precipitable material collected 

 from duplicate 0.05 ml samples on GF/C glass fiber discs 

 as described in Materials and Methods. 



