Figure 20. SPECTRAL DETERMINATION OF HA-CON A CONJUGATE 



SACCHARIDE AFFINITY CONSTANTS (K ) 



a 



Affinity constants determined as described in Materials 

 and Methods for the chromogenic ligand, PNPM. Con A 

 concentrations were 1 mg/ml and PNPM concentrations 

 varied from 0.012 ymole/ml to 0.56 ymole/ml. 



