-49- 



Post mortem dissections of female sand flies were performed as 

 soon after death as possible (usually within 12 hours) according to 

 the technique of Johnson et aj_. (1963). Data recorded for each 

 specimen included information regarding collection, sex, 

 identification, blood meals, oviposition, longevity, condition of 

 accessory glands, and presence or absence of natural parasites. 



Results 

 Species of Sand Flies Present 



An estimated 2400 sand fly specimens representing five species of 

 Lutzomyia , including one new species, were collected and eight new 

 county records established during the two years of the study (Table 

 2-1). Sand flies were taken from five of eight case sites; none were 

 collected at sites F, G, and H (San Antonio and Devine). 



Of the sampling methods used, only the Disney trap and the oil 

 traps failed to collect sand flies. Approximately 2000 flies, mostly 

 females, were taken in resting collections at Garner State Park and 

 Fawcett Boy Scout Camp. Other trapping methods also yielded mostly 

 females (Table 2-2). The ratio of male to female Lu. diabol ica in 

 resting, biting and light trap collections ranged from a high of about 

 6:10 in resting collections at Fawcett Boy Scout Camp, to around 4:10 

 in resting collections at Garner State Park. More than 350 female and 

 no male Uj. diabol ica were taken in biting collections at Garner State 

 Park. This method was not successful at other sites. At locations 

 where flies were not taken resting, CDC light traps were most 

 effective. 



