Page 



Accessory Glands and Parity 83 



Natural Parasite Infections 86 



3 LABORATORY COLONIZATION OF Lutzomyia diabolica 

 WITH NOTES ON ITS BIOLOGY IN THE LABORATORY 



(DIPTERA: PSYCHODIDAE) 91 



Introduction 91 



Materials and Methods 92 



General 92 



Immature Stages 93 



Adults 97 



Resul ts 100 



General Observations 100 



Immature Stages 103 



Adults 124 



Age-Specific Life Table 134 



Discussion and Conclusions 136 



General 136 



Immature Stages 137 



Adults 151 



Age-Specific Life Table 157 



4 EXPERIMENTAL TRANSMISSION OF Leishmania mexicana BY 

 BITES OF Lutzomyia diabolica (HALL) AND Lutzomyia 

 shannoni (DYAR) WITH NOTES ON THE EXTRINSIC 



DEVELOPMENT OF THE PARASITE 162 



Introduction 162 



Materials and Methods 163 



Infection of Sand Flies and Parasite Development 163 



Ultrastructure Studies 166 



Transmission Trials 167 



Results 168 



Infection of Sand Flies and Parasite Development.... 168 



Ultrastructure Studies 188 



Transmission Trials 190 



Discussion and Conclusions 206 



Infection of Sand Flies and Parasite Development 206 



Ultrastructure Studies 215 



Transmission of Leishmaniasis 218 



5 SUMMARY AND RECOMMENDATIONS 226 



REFERENCES 229 



BIOGRAPHICAL SKETCH 243 



VT 



