-61- 



all three had either mature or developing ova and the accessory glands 

 were full of dark granules. Of the remaining 33 females, 17 had 

 developing ova (most were about half developed) and all of these had 

 either full or partially full accessory glands. Sixteen had unde- 

 veloped ovaries, and of these, five had no granules in the accessory 

 glands; one had a very few granules; nine had accessory glands half 

 full of granules; and two had completely full accessory glands. 



Observations on the condition of the accessory glands (granule 

 formation) in another 94 dissected wild-caught females are presented 

 in Fig. 2-20. All of these females were blood-fed at the time of 

 capture or were given a blood meal immediately after capture. These 

 were compared with dissections of laboratory-reared, fed and unfed 

 females of known ages. In general, granule formation in accessory 

 glands of wild-caught sand flies paralleled ovarian development, and 

 when the ova were mature, the accessory glands were dark and full of 

 granules. Partial or complete emptying of the accessory glands often 

 occurred as eggs were deposited, but many females that deposited most 

 or all of their eggs maintained full accessory glands. Blood-fed 

 laboratory-reared flies showed basically the same trend as blood-fed 

 wild-caught flies. Unfed laboratory-reared flies either did not 

 develop granules in the accessory glands or, if they did, they 

 developed them within 24 hrs, but to a lesser degree, even after six 

 days, than seen in blood-fed females. 



Natural Parasite Infections 



The incidence of natural parasite infections found in 341 female 

 sand flies taken from south central Texas, dissected during the two 

 years of the study, is presented in Table 2-5. 



