-66- 



flies (Fig. 2-22). These could be seen slowly changing shape and 

 moving in amoeboid fashion. When present, one to three gametocysts 

 could be seen attached to the accessory glands (Fig. 2-23). The 

 eliptical oocysts were most frequently seen spilling from the 

 accessory glands or from ruptured, mature gametocysts (Fig. 2-24). 

 Oocysts were seen glued by the accessory gland material to the chorion 

 of eggs deposited by gregarine infected females. 



Microsporidians . Massive microsporidian infections were 

 discovered in the hemocoels of 3-8% of the dissected females (Fig. 

 2-25). Germination of the spores was stimulated in vitro by adding a 

 few drops of 0.2m KC1 (pH 9) to the dissecting medium (insect Ringers 

 solution) (Fig. 2-26). Ungerminated spores washed onto the larval 

 medium were fed to uninfected larvae. No infections were acquired. 



Mites . Mites were found attached to the venter and dorsum of the 

 abdomen of 14 females collected in the spring of 1982. None were 

 found on females collected in the fall of 1983. The mites were 

 tentatively identified as Eustigmaeus sp. (Berlese) (Family 

 Stigmaeidae). Scanning electron micrographs of one mite specimen are 

 presented as Figures 2-27 and 2-28. 



Other . Fungal and bacterial infections were commonly found in 

 dissections of wild-caught j.u. diabol ica . 



Discussion and Conclusions 



Determination of Sand Fly Fauna 



Many methods have been used for sampling sand fly populations. 

 Some were devised for sampling particular habitats (e.g., resting 



