-86- 



wild populations. Finally, Perkins (1982) believed that the 

 examination of accessory glands for determining parity is open to 

 question. He suggested, however, that the presence of accessory gland 

 granules in apparently nulliparous Lu. shannoni females from Florida 

 was evidence of a high probability of autogeny. The presence of 

 accessory gland granules in nulliparous, anautogenous Lu. diabol ica 

 may tend to invalidate this suggestion. 



Dissections of wild-caught and laboratory-bred female Lu. 

 diabol ica showed a wide range of variation in accessory gland 

 condition. Based on presence or absence of accessory gland granules, 

 little can be concluded from the dissections described in Fig. 2-20 

 except that those flies that died without developing ova and with no 

 evidence of granules were probably nulliparous. Based on observations 

 of laboratory-bred females, it would be virtually impossible to 

 distinguish between 4-6 day old nullipars and uni or multipars that 

 had voided the accessory glands during oviposition. One must conclude 

 that examination of accessory glands as an indicator of parity in Lu . 

 diabol ica is unreliable at best. 



Natural Parasite Infections 



Young and Lewis (1977) compiled an extensive list of accounts in 

 the literature of natural and laboratory infections of parasites in 

 Psychodidae. In their review, no records of natural parasites were 

 reported from Lu . diabol ica . This study gives the first indication of 

 several parasite infections in the species. 



Flagel lates . Keithly (1984, pers. comm.) believes that the small, 

 rounded, highly-motile flagellates found swimming in the hemocoel of 



