-93- 



Lu. diabolica are basically those described by Endris et aj_. (1982). 

 Any departures from these techniques are explained under the 

 subheadings below. 



Immature Stages 



Recovery and maintenance of eggs . Blood-fed females were kept in 

 individual or group-oviposition/rearing vials with screen-covered lids 

 until they deposited eggs and/or died. Most of the eggs were deposited 

 on the moist plaster of Paris in the bottom of the vials, but 

 occasionally a few were deposited on the plastic sides of the 

 container. These were knocked off onto the surface of the plaster 

 with a dissecting needle to prevent them from dessicating. Dead 

 adults were removed from the vials to prevent mold growth. Water was 

 added as needed to insure adequate relative humidtiy (RH) in the vials 

 during incubation. The screen lids were replaced with solid plastic 

 lids that had been perforated with a dissecting needle to allow for 

 limited gas exchange. For the first three generations, incubated eggs 

 were routinely maintained in a Hotpack® environmental chamber (Hotpack 

 Inc., Phi ladelphia, PA) at 24 + 1°C, 70 + 10% RH, 16:8 LD photoperiod, 

 but later rearing temperature was changed to 27 _+ 1°C. 



Maintenance of larvae and pupae . Individuals from a single egg 

 batch were usually reared together in the same vial. Group-rearing 

 chambers (120 ml) were also used to accomodate progeny of up to ten 

 females. Containers for rearing individual larvae were made from 24- 

 wel 1 (one larva/well) plastic tissue-culture trays with about 1/2 cm 

 plaster of Paris in each well (Perkins, 1982). A solid Plexiglas® 



