-95- 



eggs from 13 egg batches were set up in this manner and incubated at 

 either 24 + 1°C or at 27 + 1°C. 



Each vial was checked daily (AM) and the developmental progress 

 of the immatures was recorded through adult emergence. The data were 

 analyzed and compared statistically for significant differences using 

 analysis of variance (ANOVA) procedures in conjunction with the 

 student's t (z) test (Marks, 1982). 



Larval diet experiments . These experiments were designed to 

 evaluate the efficacy of various larval diets on development time and 

 survival rate. Four larval diets, listed below, were compared with 

 standard sand fly diet (Young et _al_., 1981). 



A. Standard sand fly diet, ground fine and applied dry; 



B. Standard sand fly diet mixed and incubated with liver powder 

 in a ratio of 10:1, respectively, ground fine and applied dry; 



C. Horn fly [ Haematobia irritans (Linn.)] diet (Greer and 

 Butler, 1973), consisting of sugar cane bagasse, dry mix (48 g wheat 

 flour, 36 g fish meal, 6 g sodium bicarbonate, 20 g alfalfa meal), 

 fresh cattle manure and tap water mixed in an approximate ratio of 

 1:1:5:5 by weight respectively, incubated at 27°C for 45 days, ground 

 fine and applied dry; 



D. Horn fly diet, unincubated, ground fine and applied dry; and 



E. Standard sand fly diet, coarsely ground and applied moist. 

 Diets A, B, C, and D were ground in a household coffee mill 



(Krups type 280, Robert Krups, North America, Allandale, NJ) and 

 sifted through a 40-mesh soil sieve. The life-cycle parameters of 

 larvae fed on these test diets were compared with those of individuals 

 fed on diet E, which was the same diet used in all previous colony 



