-97- 

 Adults 



Maintenance . Newly emerged adults were released into a mating 

 chamber (Endris et al_., 1982) supplied with apple slices or wedges 

 leaned against the back wall or placed on the floor of the chamber. A 

 100-ml bottle, filled with tap water and plugged with a sponge wick, 

 was placed in a corner of the chamber to help maintain the RH at or 

 above 80%. The mating chamber was kept in an environmental cabinet at 

 27 + 1°C, 70 + 10% RH, and 16:8 LD photoperiod. 



Females were usually held in the chamber for 48 to 72 hours, to 

 allow time for mating before being offered a blood meal by one of the 

 fol lowing methods: 



1. Females were captured with a tube aspirator and transfered to 

 120-ml oviposition/rearing vials provided with screen feeding lids 

 (Endris _et aj_., 1982). The exterior surface of the feeding lid was 

 held against the host's skin until the flies fed to repletion (Fig. 

 3-1). 



2. A mouse or hamster, anesthetized (IM) with ketamine chloride 

 (0.07 to 0.01 cc for mice; 0.18 to 0.20 cc for hamsters), was placed 

 in the mating chamber for up to an hour at a time. 



Hosts used for blood feeding laboratory-reared females also 

 included human, dog and rabbit. Duration of individual feedings was 

 timed by a stop watch. 



For the first two generations, males were placed with blood-fed 

 females in group or individual oviposition/rearing vials. In 

 subsequent generations, replete females were released into the mating 

 chamber with males for another 24-hr period before being placed, 

 without males, in group or individual containers. A drop of Karo® 



