-99- 



syrup and water (1:1) was placed daily on the screen lid of each vial, 

 as an energy source. 



During the first five colony generations, the screen-lid vials 

 containing gravid females were kept in an open tray inside the 

 environmental chamber. Because the air inside the chamber was 

 continuously circulated by a fan, the plaster in the vials dried out 

 within one or two days, resulting in an unsatisfactory surface for 

 oviposition. To prevent this excessive drying in subsequent 

 generations, moist paper towels were placed in the bottom of the open 

 tray and the entire tray was enveloped in a clear plastic bag. This 

 innovation maintained the RH in the holding environment for gravid 

 females at or near 100%. 



About 10% of the females that survived oviposition were offered a 

 second blood meal to see if they would complete a second gonotrophic 

 cycle. 



Longevity experiments . Newly emerged individuals (14th 

 generation) of each sex, from vials in which no adults of the opposite 

 sex had eclosed, were held in clean oviposition/rearing vials at 27°C 

 and near 100% RH until death. A drop of Karo® syrup, placed daily on 

 the surface of each screen lid, provided the sole source of nutrient. 



Autogeny experiment . Thirty newly emerged, 14th-generation 

 females were paired with newly emerged males in 7-dram 

 oviposition/rearing vials and held at 27°C and near 100% RH until 

 death. They were checked daily for signs of autogenous egg 

 production. Each day a drop of Karo® syrup was placed on the screen 

 lid of each vial as the sole source of nutrient. The females were 

 dissected post mortem to determine if mature, undeposited eggs had 

 developed. 



