-103- 



The time of development from engorgement to first emergence of 

 adults of the succeeding generation (generation time) averaged 66 days 

 for generations 1 through 9, with a range of 49 to 92 days (Fig. 3-4). 

 With changes in handling procedures and rearing conditions, and 

 possibly some genetic adaptation of the colony, the mean generation 

 time dropped to about 32 days in the 13th generation. 



Immature Stages 



Eggs . The eggs of J_u. diabolica have a characteristic morphology 

 which distinguishes them from other Lutzomyia eggs (Endris, 1982). At 

 oviposition they are white to gray in color, but turn shiny dark-brown 

 or black within a few hours. They are banana-shaped and measure about 

 0.35 x 0.1 mm. The thick shells retain their shape after hatching, 

 often with little more than a faint slit to betray the first instar's 

 emergence. 



Table 3-1 summarizes fecundity and hatching observed in 

 generations 1 through 9 and 13. Egg batches ranged in size from one 

 to 76, with a mean batch size of about 27 eggs. One wild-caught 

 female laid 84 eggs and one F^g female laid 88 eggs. Neither of these 

 was included in the table. The largest mean number of eggs per batch 

 in a given generation was 36.1 (5th generation) and the smallest was 

 24.9 (3rd generation). 



Incubation time ranged from 5 to 113 days in the laboratory, with 

 an overall mean of 8 days. First generation eggs hatched in 1-1/2 to 

 3 days less time than those of the 2nd and 3rd generations. At the 

 beginning of the 4th generation the colony rearing temperature was 

 raised from 24°C to 27°C, resulting in an average reduction in 



