-112- 



Table 3-3. Duration of larval and pupal stages in generations 1 thru 



9 and 13 of a laboratory colony of Lutzomyia diabol ica main- 

 tained at 24 ± 1°C and 70 ± 10%RH for the first three 

 generations and 27 ± 1°C and 70 ± 10%RH for all subsequent 

 generations. 



Number 

 of 



Larval Stage in Days 

 (1st thru 4th instar) 



Pupal Stage in Days 



Generation 



Broods 



x± SD (range) 



x± SD 



grange) 



1 



60 



27. 7± 6.4(18-50) 



8.2±2.3 



[5-22) 



2 



55 



37.7±24.4(17-163) 1 



9.5±8.1 



[5-67) 



3 



45 



49. 4±29. 3(23-151) 



10.2±4.3 



'4-28) 



4 



40 



31. 9± 6.5(21-47) 1 



8.0±2.4 



[3-16) 1 



5 



42 



31. 5± 7.4(23-49) 



7.1+2.2 



[4-14) 



6 



23 



21.3+ 4.4(15-33) 



6.7±1.1 



[5-8) 



7 



6 



38. 8±20. 7(20-65) 



9.0±5.6 



'6-19) 



8 



27 



53. 4± 8.9(35-71) 



8.0±2.5 



4-20) 



9 2 



13 



45. 1± 9.4(35-72) 



7.6±4.0 



4-20) 



13 



25 



17. 3± 2.3(14-23) 1 



6.2±0.51 



5-7) 1 



Overall 



336 



35.9 (14-163) 



8.4 



3-67) 



1 = Significantly different from preceding value (AN0VA procedures, 



PR > F = 0.0001; Duncan's multiple range test, a = 0.05). 



2 = In the 9th generation the colony was rejuvenated by the addition 



of 76 egg batches from wild-caught females. 



