-129- 



significantly more males were produced on diet E (standard sand fly 

 diet, coarsely ground, applied moist) (ANOVA procedures, PR>F = 

 0.0001; Duncan's multiple range test, a = 0.05). 



Longevity . The mean longevity of 30 virgin males of the 13th 

 generation, held in individual oviposition/rearing vials at 27°C and 

 near 100% RH, was 14.9 days (range = 4 to 20 days). The mean longevity 

 of virgin females of the same generation, held under the same conditions, 

 was 11.7 days (range = 4 to 21 days). Longevity of females that took 

 a single blood meal was 10.1 days for all generations and ranged from 

 5 to 38 days (Table 3-6). Postov iposition longevity averaged about 1 

 day for all generations and ranged from to 29 days (Table 3-6). 

 Females that survived oviposition and which took a second blood meal 

 lived an average of 12.3 days (n = 47), or about two days longer than 

 singly fed females. 



Mating . Males emerged with genitalia inverted 180° from the 

 normal position and were not reproductively mature until their 

 genitalia had rotated, about 12 to 24 hrs posted osion. Females seemed 

 to be reproductively mature at the time of eel osion and were seen 

 mating immediately after being released into the mating chamber. 

 Mating was observed at all hours of the day, before, during, and after 

 blood feeding, but usually after. Copulation lasted from three to five 

 minutes, but was as short as 47 sec and as long as 14 min 23 sec (n = 

 16). During the early part of the 1st generation, males were paired 

 with blood-fed females for life in individual oviposition/rearing 

 containers to insure maximum opportunity for insemination. It was 

 later discovered that equivalent mating success could be achieved by 

 releasing blood-fed females into the mating chamber for an additional 



