-136- 



based on data obtained from several generations but principally from 

 a cohort of individuals produced by 32, 12th-generation females that 

 emerged together and fed on the same day. 



Discussion and Conclusions 

 General 



Johnson and Hertig (1961) discovered that when sand fly cultures 

 were moved from one room at 25.5°C to another at 26.5°C, certain 

 species, which were not doing well in the first room, began to thrive, 

 and that the "hardier" species fared better at even higher 

 temperatures. Gemetchu (1976) attributed much of the success of a 

 colony of P. longipes Parrot and Martin to changes in the quality of 

 larval diet provided. Kil 1 ick-Kendrick _et a_L (1977) observed an 

 approximate 25% reduction in generation time of Lu. 1 ongipal pis (Lutz 

 and Neiva) due to a change from an open insectary, with less constant 

 conditions, to environmental cabinets with controlled moisture. Ward 

 (1977) observed a ten-fold increase in numbers of adult Lu . 

 flaviscutellata (Mangabeira) from the 6th to the 9th colony generation 

 as a result of adaptation to exclusive feeding on hamsters and 

 increased refeeding. 



The most significant factors contributing to the success of this 

 laboratory colony of Lu. diabol ica were 1) a large initiatory stock 

 of eggs and timely rejuvenation with additional wild-stock, insuring 

 adequate numbers of adults for mating and reproduction, in spite of 

 less than optimal rearing conditions; 2) a change in rearing 

 temperature from 24°C to 27°C; 3) strict regulation of moisture in 



