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oviposition/rearing vials; 4) improvements in larval diet; and 5) 

 genetic selection, since each successive generation was set up with 

 flies emerging from the preceding generation (see Kil lick-Kendrick et 

 a! ., 1977). It is impossible at this point to identify the specific 

 effects of infusion of the original inbred colony stock with wild 

 material. Two possibilities exist; either the original genetic stock 

 was completely or partially replaced by the new material, or the new 

 material was assimilated into the existing colony. This latter 

 possibility would likely have reversed, at least temporarily, much of 

 the genetic selection that occurred in previous colony generations. 

 The original inbred stock may have selected for low survival, a trend 

 turned around by the infusion with wild stock. 



From the colony's inception, frequent changes and adjustments in 

 handling procedures and rearing conditions were essential to meet the 

 specific needs of the subject population. Initial changes amounted to 

 little more than guesswork, but through trial and error the guesswork 

 became educated and fine-tuning of the colony to acceptable levels of 

 productivity was possible. 



Immature Stages 



Eggs . Lindquist (1936) first described the eggs of Lu . 

 diabol ica , saying that they possessed slightlty branched, longitudinal 

 striations on their surfaces. Endris (1982) examined the eggs with an 

 electron microscope and described the surface topography as a series 

 of discontinuous, paral lei- longitudinal ridges that are not laterally 

 connected. He proposed a scheme for classification of 41 species of 

 New World sand flies based on the distinctive surface topographies of 



