-190- 



cl earl y visible. The number of microtubules averaged 86.5 and ranged 

 from 49 to 151 (n = 62), increasing in number with the diameter of the 

 parasite. Conspicuous organelles included the flagellum (F) emerging 

 eccentrically from the flagellar reservoir (FR), kinetoplast (K), 

 mitochondrion (M), golgi apparatus (G), nucleus (N), and dense 

 inclusion vesicles (DV) (Fig. 4-14 and 4-15; see Molyneux et al., 

 1975). 



The flagellum of the promastigote is surrounded by a somewhat 

 corrugated flagellar sheath (FS) (Fig. 4-15). Associated with the 

 flagellar reservoir are four microtubules (RT) which run parallel to 

 its axis (Fig. 4-14). They have the same diameter as the 

 subpel 1 icular microtubules. The axoneme (AX) of the flagellum has a 

 typical nine-plus-two configuration (Fig. 4-14). The flagella of 

 promastigotes in the abdominal midgut were commonly associated with 

 the microvi 1 1 i (MV) 1 ining the gut wal 1 , although no points of 

 attachment were seen. In the region of the stomodeal valve, where 

 there are no microvilli, many broad promastigotes were attached to the 

 cuticular lining (CT) of the valve by foot-like enlargements, or 

 hemidesmosomes (HD), at the tips of their flagella (Fig. 4-15; see 

 Kil lick-Kendrick et aj_., 1974). 



Transmission Trials 



Five (24%) of 21 hamsters were infected by bites of 25 potentially 

 infected Lu. diabol ica (Table 4-7). Of the successful trials, four 

 were single feedings and one was a double feeding. The four flies 

 involved in single feeding transmissions took partial blood meals and 

 the two involved in the double feeding fed to repletion. 



