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of a hamster's right ear. Four other lesions appeared shortly 

 thereafter, corresponding to the sites of the other infective bites. 



Similarly, in the second transmission trial, 15 potentially 

 infected flies probed or fed on the ears of an uninfected hamster six 

 to eight days after the infecting blood meal. This trial resulted in 

 eight lesions, four on each ear, appearing within 30 days. Seven of 

 the flies probed only, eight took full or partial blood meals, and 

 several that took blood also probed first at different locations 

 before inserting their mouthparts. 



The remaining three trials with Lu. shannoni were individual 

 feedings, where only one potentially infected female was allowed to 

 feed, five to eight days after the infecting meal, each on an 

 uninfected hamster. All three took partial blood meals and all bites 

 resulted in transmission of Leishmania , with lesions appearing between 

 22 and 43 days (Table 4-9). Two of these latter transmissions were 

 from bites that drew only a trace of blood. The mean interval between 

 first and second blood meals of Lu. shannoni involved in these 

 transmissions was 6.9 days, and ranged from five to eight days. Table 

 4-10 shows the results of postfeeding dissections of female Lu. 

 shannoni involved in the transmissions. 



No transmissions were obtained in limited refeeding of flies 

 infected with L m. amazonensis or L _b. guyanensis . Results of 

 trials with L d_. infantum will be forthcoming due to the long 

 incubation period of the parasite in hamsters. 



