28 



visited by snails); ambulatory areas (areas visited by snails without 

 feeding); and grazing areas (areas visited by snails where feeding had 

 occurred). Each of the labeled samples were placed into a solution of 

 50% Gluteralaldahyde and 50% phosphate buffer at pH 7.0 for two hours 

 (Anderson, 1966) and then reduced to 2 x 2 x .05 mm samples. Samples 

 were left in osmium for two additional hours, removed, washed, and 

 placed into a phosphate buffer (pH 7.0) for approximately 18 hours. 



The following day the samples were subjected to both an acetone 

 dilution series and a Freon TF dilution series which consisted of 5, 

 10, 20, 30, 40, 50, 50, 70, 80, 90, 95, 100, 100 , 100 % washes for ten 

 minutes each. Samples were critically point dried using a Freon 13 

 critical point dryer. 



The specimens were placed on one centimeter cylindrical studs and 

 held in place by a conducive silver glue. A Hummer II sputter coater 

 gold plated the sample's surface. A JEOL/JSM-35 scanning scope was 

 used to review and to photograph the surface. Surface photographs were 

 taken with a polaroid camera horizontally mounted to the scanning 

 electron microscope. 

 Results and Discussion 



Control areas not affected by the citrus tree snal are covered 

 generally with microbiota (Figures 4-9). The ambulatory areas demon- 

 strated the ability of D. dormani to encrust with mucilage the surface of 

 the fruit. Spermatophores were pressed to the surface and their sperm 

 sacs ruptured. Mycelia were encompassed generally within the mucilage 

 (Figures 10-17). Grazed areas, one centimeter wide, were void of micro- 

 biota and were covered generally with a thin mucilage veil (Figures 

 18-22). 



