45 



sexual pupae. The eyes of the sexual pupae appear to be 

 less affected than the eye of the worker pupa; this may 

 be an artifact due to the larger (ca 16-20X in area) eyes 

 of sexual pupae. The diseased worker pupa is in an advanced 

 stage of infection, and exhibits typical pathognomonic signs. 

 Note the irregular outline of the eye, the derangement of 

 the facets, and its fainter color. 



The development of pathognomonic signs is recorded 

 in Fig 9. In the first pair of photographs, the healthy 

 and diseased pupae (IH and ID, respectively) are almost 

 indistinguishable. The diseased pupa exhibits a faint clearing 

 of the petiole, and in life--but not in the photograph-- 

 the earliest faint signs of eye teratology could be detected. 

 In the photograph taken 48 hours later (3D) , the clearing 

 in the petiole is not evident due to a slight change in 

 lighting. Subsequent photographs show the development of 

 clearing in the head and petiole. The eye of the diseased 

 pupa is rather blurry in these photographs. This is not 

 due to focus, but to a fluid space over the eye. 



The healthy pupa eclosed on day 18. The last photo- 

 graph of the diseased pupa (3D) shows an indentation in 

 the gaster, indicating dehydration. In a colony, rupture 

 would probably have occurred by this time as a result of 

 manipulation (being moved, groomed, etc.) by the workers. 



Frontal sections of whole heads of younger and older 

 diseased pupae are presented, respectively, in Figs 10 and 12. 



