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aqueous honey solution. A diet composed of housefly or stable- 

 fly pupae (1 liter) pureed with cooked ground beef (ca 250 g) 

 in a blender and thoroughly mixed with whole eggs (1 dozen) 

 and vitamins (5.0 ml Poly Vi-Sol, Mead Johnson & Co., Evansville, 

 Indiana 47721) in hot liquid agar solution (50 g agar dissolved 

 in 1.5 L distilled water) was also used as a supplement 

 when insects were in short supply. The warm liquid diet 

 was poured to a depth of ca 1.5 cm in shallow enamel pans 

 and allowed to gel. It was then cut into cubes for dispens- 

 ing to the ants. The excess diet was covered with plastic 

 film and frozen or stored in the refrigerator. 



Laboratory Propagation of Burenella dimorpha 

 Colonies of ants were infected with B. dimorpha by 

 feeding them boiled egg yolk wetted with a suspension of 

 spores to a consistency of paste. These colonies were then 

 maintained and diseased pupae were harvested periodically 

 as spores were needed. Prior to infection, colonies were 

 carefully examined for other diseases using the methods 

 described by Jouvenaz et al. (1977). 



Harvest of Spores 

 Suspensions of spores free of cellular debris were 

 obtained by density gradient centrifugation using Percoll 

 (Pharmacia Fine Chemicals, 800 Centennial Ave., Piscataway, 

 N.J. 08854). Pupae exhibiting signs of advanced infection 



